Anti-Rad51 Antibody

The Anti-Rad51 Antibody from Antibodies.com is an agarose-conjugated chicken polyclonal reagent raised against the Rad51 recombinase, a central effector of homologous recombination (HR) and DNA double-strand break repair. Supplied as 100 µl of liquid IgY coupled to agarose beads in a ready-to-use vial, it enables direct capture of native Rad51 complexes without secondary reagents, simplifying chromatin and nuclear extract workflows. Validated for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP), the antibody recognizes Rad51 in human, mouse, rat, and chicken samples, supporting comparative repair studies across vertebrate models. The IgY isotype avoids interference from mammalian Fc-binding proteins such as Protein A, Protein G, and rheumatoid factor, reducing background in pull-downs from rodent lysates. For best results, store the conjugate refrigerated at 4 °C, do not freeze the agarose slurry, and avoid repeated freeze-thaw cycles by aliquoting on first use; gently resuspend before pipetting to keep beads in suspension. Working bead volumes range from 10 to 40 µl per mg of clarified lysate, depending on Rad51 expression levels in the starting material, and the product is intended for research use only.