Anti-Rad23B (5H1) Antibody

Rad23B functions as a nucleotide excision repair factor and ubiquitin-receptor that shuttles damaged-DNA substrates toward the proteasome, and this unconjugated mouse monoclonal reagent offers a defined tool to track that biology. Clone 5H1, raised in mouse, recognizes endogenous Rad23B in human, mouse, rat, and monkey specimens, allowing Western blot, immunocytochemistry, and immunohistochemistry workflows to be run from a single antibody lot. Antibodies.com provides the immunoglobulin as 100 µl of purified protein in 0.1M Tris-Glycine buffer at pH 7.4, supplemented with 150 mM sodium chloride, 0.2% sodium azide, and 50% glycerol, which dilutes cleanly into standard blocking, wash, and incubation buffers. Because the reagent is unconjugated, laboratories can pair it with HRP, biotin, or fluorophore-tagged secondary antibodies appropriate to their preferred detection chemistry, including chromogenic Western blot membranes, fixed-cell imaging, and antigen retrieval-based tissue sections. Investigators studying UV-induced lesion repair, XPC complex recruitment, or proteasomal substrate turnover routinely use this clone to follow Rad23B abundance and subcellular localization across whole-cell lysates and paraffin-embedded material. Storage at refrigerated temperatures with 50% glycerol as a cryoprotectant preserves activity, and aliquoting on first use limits repeated freeze-thaw cycles for long-term stability.