Anti-RAD18 Antibody

This Antibodies.com primary antibody is a rabbit polyclonal antibody to RAD18. It is unconjugated and reacts with human samples. WB is a listed application; manufacturer records also identify IP and IF, with validation stated for WB, ICC/IF and IP.

Manufacturer documentation describes a synthesized peptide derived from an internal region of human RAD18, while another specification identifies a GST-tagged synthetic peptide containing the 113 carboxyl-terminal amino acids of human Rad18 protein. The antibody detects endogenous levels of total RAD18 protein and is affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The stated concentration is 1.0 mg/mL, and the recommended WB dilution is 1:1,000.

The product form is liquid. A stated formulation is rabbit IgG in phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol; another record lists phosphate-buffered saline with 50% glycerol. It is shipped at 4°C. Upon delivery, aliquot and store at -20°C, avoiding freeze/thaw cycles.