Anti-RAD18 Antibody

Raised in rabbit and supplied as an unconjugated 100 µl preparation, this primary antibody from Antibodies.com targets the human RAD18 protein, an E3 ubiquitin-protein ligase central to the post-replication repair of UV-damaged DNA. RAD18 monoubiquitinates PCNA at Lys164 to recruit error-prone translesion synthesis polymerases past stalled replication forks, and the antibody is validated for western blot and immunohistochemistry on human samples. It is commonly used to investigate replication stress, lesion bypass signaling, and the recruitment of repair factors to sites of damage. The polyclonal reagent supports detection of endogenous RAD18 expression in fixed tissue sections and in cell or tissue lysates, complementing studies of genome stability, Fanconi anemia pathway crosstalk, and tumor cell responses to DNA-damaging agents such as UV light, cisplatin, or hydroxyurea.