Anti-RAD1 Antibody

From Antibodies.com, this primary antibody is supplied as a 50 µl aliquot of rabbit polyclonal antiserum raised against a recombinant human RAD1 protein and affinity-purified on the immunogen. The reagent is unconjugated and formulated at 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol; the IgG isotype preparation is shipped on cold packs at 4 °C and should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. The antibody recognizes the endogenous RAD1 checkpoint protein (also known as hRAD1, Rad1-like DNA damage checkpoint protein, and REC1) and has been validated for Western blotting, with immunofluorescence reported as an additional supported application. Cross-reactivity has been demonstrated against human, mouse, and rat samples, making the reagent suitable for comparative cell-cycle and DNA-damage response studies across these species. Intended strictly for research use, this polyclonal reagent provides a versatile tool for laboratories investigating the RAD9–RAD1–HUS1 (9-1-1) complex and related genome-surveillance pathways.