Anti-RAD Antibody

The Antibodies.com polyclonal reagent targets the RAD DNA repair protein, a RecA-family factor essential for homologous recombination and the cellular response to double-strand breaks. Raised in rabbit and supplied as an unconjugated antiserum at 100 µl per vial, it is validated for western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples.

RAD operates within the BCDX2 complex alongside XRCC2, XRCC3, and DMC1 at replication-fork damage sites, where its ATP-dependent strand-exchange activity supports strand invasion and Holliday junction processing. Investigators characterizing Fanconi anemia pathway crosstalk, BRCA1-deficient tumor models, and PARP-inhibitor sensitization frequently use this reagent to monitor RAD foci formation at laser-induced DNA lesions and to compare expression in repair-proficient versus repair-deficient cell lines.

Buffer composition includes standard stabilizers suitable for short-term storage at 4 °C and long-term aliquoting to avoid repeated freeze-thaw cycles. Each lot is quality-controlled against full-length RAD in human and rodent lysates, making it a useful tool for DNA damage response studies, meiotic recombination research, and genomic stability screens in research use only settings.