Anti-Rab9 Antibody

Produced in rabbit as a recombinant monoclonal antibody from Antibodies.com, this unconjugated primary reagent specifically targets the Rab9 small GTPase, a key regulator of late endosome-to-trans-Golgi trafficking and mannose-6-phosphate receptor recycling. Validated for Western blot and immunocytochemistry/immunofluorescence, the antibody reacts with human, mouse, and rat samples, supporting comparative cell biology studies across multiple model systems.

Each vial delivers 100 µL of antibody in TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, providing a stable format for direct conjugation or use in standard immunodetection workflows. The reagent enables probing of endogenous Rab9 expression and localization in cultured mammalian cells, primary tissue lysates, and fixed specimens for studies of endosomal sorting, autophagy regulation, and Golgi-associated vesicular transport.

Rab9 operates in the trafficking pathway that recovers mannose-6-phosphate receptors from late endosomes back to the trans-Golgi network, and is implicated in a non-canonical autophagy route that functions independently of the ATG5 and ATG7 conjugation machinery. The antibody is therefore used in research on membrane trafficking dynamics, lysosomal biogenesis, and cargo sorting relevant to neurodegeneration, infectious disease mechanisms, and cancer cell biology contexts.