Anti-RAB8B Antibody

RAB8B is a small GTPase within the broader Rab family that governs vesicular transport between the trans-Golgi network, basolateral recycling compartments, and the plasma membrane. This unconjugated rabbit polyclonal antibody from Antibodies.com is validated for Western blot detection in lysates from human, mouse, and rat samples, supporting comparative studies of Golgi-associated sorting, basolateral trafficking in polarized epithelia, and membrane delivery during neurite extension.

The reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, at approximately pH 7.2. Each vial provides 100 µl of antibody solution, and the polyclonal format binds multiple epitopes on RAB8B, which can increase signal robustness across heterogeneous sample preparations. Researchers studying cancer cell migration, neuronal development, and metabolic membrane trafficking use anti-RAB8B antibodies to probe expression levels and subcellular distribution.

RAB8B localizes to the trans-Golgi network, pericentriolar recycling endosomes, and basolateral membrane regions, where it coordinates vesicle docking through effectors such as optineurin and RABIF. For Western blot, working dilutions in the range of 1:500 to 1:2000 are commonly used depending on target abundance and detection chemistry. The product should be stored refrigerated and protected from light to preserve binding activity across its working shelf life.