Anti-RAB8B Antibody

This unconjugated rabbit polyclonal IgG from Antibodies.com targets the Ras-related protein Rab-8B (RAB8B), a 23.6 kDa monomeric GTPase of the Rab family that regulates post-Golgi vesicular trafficking. Raised against the human RAB8B sequence, the reagent is validated for Western blot detection of endogenous protein in human, mouse, and rat sample lysates and is supplied as 50 µl of liquid in a single vial. For storage, aliquot the antibody and store at -20 °C to limit freeze-thaw cycles and preserve activity across multiple immunoblotting experiments.

RAB8B shares 96% amino acid identity with the paralog RAB8A but carries out distinct trafficking roles, including polarized membrane delivery from the trans-Golgi network, primary cilium formation, regulated GLUT4 surface expression in insulin-responsive tissues, and dynamic association with Sertoli cell junctions during spermatogenesis. The unconjugated format permits flexible pairing with HRP- or fluorophore-conjugated secondary reagents and supports chemiluminescent or fluorescence-based readouts in cell biology and neuroscience research.

Investigators detect a band near the predicted 23.6 kDa molecular weight of full-length human RAB8B following SDS-PAGE separation and transfer onto nitrocellulose or PVDF membranes, making this reagent a useful reagent for studies of vesicular transport, ciliogenesis-associated disorders, metabolic regulation in muscle and adipose tissue, and tumor cell migration. For research use only.