Anti-RAB8B Antibody

RAB8B is a small Ras-related GTPase of the Rab family that coordinates post-Golgi vesicle trafficking, polarized exocytosis, and retrograde transport from endosomes back to the trans-Golgi network, and this polyclonal reagent from Antibodies.com is raised in rabbit against the human protein for immunoblotting workflows. The unconjugated IgG is supplied as a 100 µl liquid aliquot in a vial and is validated for Western blot detection of endogenous RAB8B in lysates prepared from human, mouse, and rat samples, with cross-reactivity confirmed across these three species so that findings can be translated between mouse, rat, and human model systems. Published work on Rab8a and Rab8b double-knockout mice shows that RAB8B acts redundantly with the closely related RAB8A to mediate apical cargo delivery, while basolateral and dendritic trafficking remain unaffected, and the same studies demonstrate that RAB8B cooperates with Rab10 in ciliogenesis when both Rab8 isoforms are absent. The reagent is therefore relevant to research on epithelial polarity, primary cilia assembly, and the trafficking of receptors and transporters such as GLUT1 and GLUT4 that depend on Rab8-family effectors at the pericentrosomal recycling compartment. Storage at -20 °C in working aliquots is recommended to limit freeze-thaw cycles, and the unconjugated format allows flexible pairing with HRP-, fluorophore-, or biotin-conjugated secondary antibodies depending on the detection platform. The reagent is supplied for research use only and is backed by batch documentation to support reproducible immunoblotting across experiments.