Anti-RAB5A Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against RAB5A, a small GTPase of the Rab family that acts as a master regulator of early endosome biogenesis, homotypic endosome fusion, and clathrin-mediated endocytosis. The unconjugated reagent is supplied in a 100 µl vial of polyclonal sera and is validated for western blot, immunohistochemistry, and immunofluorescence workflows, enabling detection of the approximately 24 kDa GTPase across human, mouse, and rat sample sets.

The target itself is biologically distinctive: RAB5A cycles between an active GTP-bound membrane-associated state and an inactive GDP-bound cytosolic state, recruiting effectors such as EEA1 and the PI3K subunit hVPS34 to early endosomes where it drives endosomal maturation, EGFR internalization, and downstream signaling from internalized receptors. Researchers studying endocytic trafficking, receptor downregulation, or the early endosomal compartment rely on this reagent to visualize subcellular distribution and quantify expression changes in cultured mammalian cells and in tissue sections.

As an unconjugated primary antibody, the format is compatible with the secondary detection system of the user's choice, supporting flexible integration into existing antibody panels for fluorescence microscopy, chromogenic IHC, or chemiluminescent western blotting studies of vesicle trafficking and receptor-mediated endocytosis.