Anti-RAB12 Antibody

RAB12 is a small GTPase in the Rab family that cycles between an inactive GDP-bound state and an active GTP-bound state, directing vesicle transport and tethering events within the endolysosomal system. The unconjugated rabbit polyclonal IgG reagent from Antibodies.com detects endogenous human and mouse RAB12 by western blot, supporting research into autophagy regulation, aggrephagy, and nutrient-responsive signaling through mTORC1.

Supplied as a 100 µl vial in liquid format for research use only, the antibody is recommended for use on PVDF or nitrocellulose membranes with chemiluminescent or fluorescent detection. For continuous use, store at -20 °C, aliquot to limit freeze-thaw exposure, and titrate working dilutions against the lysate or tissue preparation of interest. The polyclonal format provides broader epitope coverage than a monoclonal reagent, which is useful when probing endogenous RAB12 levels across multiple cell lines or primary tissue samples.

Because RAB12 has been linked to alpha-synuclein turnover and protein aggregate clearance in neuronal models, this reagent pairs naturally with lysosomal and autophagy marker antibodies in comparative western blot studies of Parkinson's and other neurodegenerative disease models. Endogenous detection on human and mouse samples makes the antibody appropriate for translational work spanning rodent disease models and human cell systems.