Anti-Rab 5A Antibody

Manufactured by Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against a synthesized peptide derived from the internal region of human Rab 5A, a small GTPase involved in early endosome trafficking and membrane fusion. The unconjugated antibody is supplied as a 50 µL liquid formulation in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide at a working concentration of 1 mg/mL, and it detects endogenous levels of Rab 5A protein in samples from human, mouse, and rat. Validated applications include Western blot (WB) and ELISA, making the reagent suitable for both signal-detection immunoassays and quantitative binding studies across multiple species. The antibody is shipped at 4 °C and should be aliquoted upon arrival and stored at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Affinity-chromatography purification using the epitope-specific immunogen ensures batch-to-batch consistency, while compatibility with common goat anti-rabbit IgG H&L secondaries (HRP, FITC, biotin, and AP conjugates) gives researchers flexibility in detection chemistry for endocytic and vesicular-transport investigations.