Anti-Pyruvate Dehydrogenase Antibody

For researchers studying mitochondrial pyruvate metabolism, this Antibodies.com monoclonal reagent targets the C-terminal region of Pyruvate Dehydrogenase. Supplied as 100 µl of purified mouse monoclonal immunoglobulin in 0.1M Tris-Glycine buffer (pH 7.4, 150 mM NaCl) with 0.2% sodium azide and 50% glycerol, the unconjugated preparation is validated for western blot and immunocytochemistry in human and mouse samples.

The antibody binds the C-terminal epitope of the PDH complex, making it a useful reagent for investigating TCA cycle entry, acetyl-CoA production, and the regulation of carbohydrate oxidation. Researchers commonly apply this clone to whole-cell lysates for band detection by WB and to fixed cell preparations for ICC staining, where it is paired with a labeled secondary antibody.

Storage in the glycerol-containing buffer at -20°C preserves activity across multiple freeze-thaw cycles, and the included sodium azide prevents microbial contamination of the vial during repeated pipetting.