Anti-PXN (phospho Y31) Antibody

Paxillin (PXN) serves as a scaffold at focal adhesions, linking integrin engagement to the actin cytoskeleton and downstream signaling cascades. Tyrosine 31 phosphorylation, in particular, marks an active signaling state induced by Src family kinases following integrin ligation or growth factor stimulation, and tracking this modification is widely used in studies of cell migration, adhesion turnover, and metastatic behavior. The reagent from Antibodies.com is a rabbit polyclonal IgG raised against the phosphorylated Y31 epitope of human paxillin, supplied as 50 µl of unconjugated antiserum in a single-use vial, suitable for western blot detection of the phosphoprotein band. Cross-reactivity has been validated against human, mouse, and rat samples, making it appropriate for comparative work across cultured mammalian cell lines and primary tissue lysates. Researchers probing focal adhesion dynamics, integrin signaling, or oncogenic transformation pathways typically pair this reagent with total paxillin antibodies to assess site-specific phosphorylation stoichiometry. For best results, storage at -20 degrees C in working aliquots preserves antibody activity across repeated freeze-thaw cycles, protecting the phospho-specific binding site from dephosphorylation and degradation.