Anti-PVALB Antibody

Parvalbumin (PVALB) is a small EF-hand calcium-binding protein that buffers intracellular calcium in fast-contracting skeletal muscle fibers and in a defined subset of GABAergic interneurons, including chandelier and basket cells of the cerebral cortex. This unconjugated rabbit polyclonal antibody from Antibodies.com is supplied as 100 µl of liquid at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.3, with reactivity confirmed against human, mouse, and rat samples in western blot applications.

The antibody detects endogenous parvalbumin, which migrates near 12 kDa on SDS-PAGE gels, matching the expected position of the mature protein in skeletal muscle homogenates and brain lysates. Researchers commonly use parvalbumin as a marker to distinguish fast-twitch glycolytic fibers from slow-twitch oxidative fibers in muscle physiology studies, to map inhibitory circuit development in neuroscience, and to verify GABAergic interneuron identity in single-cell transcriptomic datasets.

For western blot workflows, the reagent is typically diluted in blocking buffer and detected with HRP-conjugated anti-rabbit secondary antibodies and chemiluminescent substrate. The vial contains 0.05% sodium azide as a microbial preservative, so appropriate laboratory safety practices should be observed during handling. To preserve IgG integrity across multiple experiments, aliquot the antibody upon arrival to limit freeze-thaw cycles of the stock solution.

This reagent is intended for research use only and supports studies of neuromuscular pathology, interneuron development in psychiatric and neurodegenerative disease models, and fiber-type composition analysis in exercise physiology and metabolic research.