Anti-PTTG1 Antibody

PTTG1 (Pituitary Tumor-Transformating Gene 1), also known as securin, is a key regulator of sister chromatid separation during mitosis, where it binds and inhibits separase until anaphase onset. This Rabbit polyclonal antibody from Antibodies.com is raised against human PTTG1 and validated for Western blot and immunofluorescence workflows.

The unconjugated reagent is supplied as 100 µl of polyclonal antiserum, supporting research into cell cycle progression, chromosome segregation, and tumor biology. PTTG1 is frequently overexpressed in pituitary, thyroid, breast, and colorectal tumors, making it relevant for cancer research and studies of genomic instability. The antibody specifically recognizes the human PTTG1 protein, enabling assessment of endogenous expression levels by Western blot and subcellular distribution by immunofluorescence in cultured human cell lines.

On Western blot membranes the antibody detects PTTG1 in whole cell lysates, while immunofluorescence staining highlights mitotic figures, where securin concentrates on condensed chromatin and at centrosomes until separase is activated at the metaphase-to-anaphase transition. Store the vial at -20 °C in small aliquots and avoid repeated freeze-thaw cycles to preserve reagent performance. This reagent is intended for research use only.