Anti-PTPRN Antibody

This rabbit polyclonal antibody from Antibodies.com targets PTPRN (protein tyrosine phosphatase receptor type N), also known as IA-2, a transmembrane dense-core granule protein expressed in neuroendocrine cells and pancreatic beta-cell islets. Raised in rabbit and supplied as an unconjugated preparation at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the reagent is validated for Western blot and immunohistochemistry on human, mouse, and rat samples. Each vial contains 100 µL of polyclonal antiserum, suitable for detection of this receptor-type phosphatase in studies of islet autoimmunity, insulinoma characterization, and neuroscience research on secretory granule proteins.

Because PTPRN is a well-established autoantigen implicated in Type 1 diabetes, this reagent is widely cited in studies of islet autoantibody panels and beta-cell mass analysis. It is also used to examine the subcellular distribution of dense-core vesicle components in pituitary and adrenal chromaffin cells, where the phosphatase co-localizes with hormones and chromogranins. As an unconjugated primary antibody, it is compatible with a broad range of user-selected secondary detection reagents, including HRP-, biotin-, or fluorophore-conjugated secondaries for chromogenic, amplified, or immunofluorescence workflows.

For best results, the antibody should be stored refrigerated in its original vial, aliquoted to limit freeze-thaw cycles, and protected from prolonged exposure to light. Working dilutions should be titrated empirically for each application; typical Western blot loadings start in the 1 to 5 µg/ml range, while immunohistochemistry on paraffin-embedded sections generally requires higher concentrations and an appropriate antigen retrieval step. The polyclonal format recognizes multiple epitopes on PTPRN, providing robust signal across denaturing and lightly fixed preparations and offering a useful option when monoclonal clones fail to detect lower-abundance targets in tissue sections.