Anti-PTPRC Antibody

PTPRC, also known as CD45 or leukocyte common antigen, is a receptor-type protein tyrosine phosphatase broadly expressed across the hematopoietic lineage, where it regulates lymphocyte signaling through dephosphorylation of Src family kinases. Antibodies.com supplies this unconjugated rabbit polyclonal reagent for researchers probing pan-leukocyte identity in human and mouse samples.

The 50 µL format supports multiple experimental workflows including western blot analysis of lymphoid tissue lysates, immunoprecipitation of phosphatase complexes, immunohistochemistry for visualizing immune cell infiltrates in tumor microenvironment studies, and flow cytometry for quantifying immune cell populations. The polyclonal IgG backbone broadens epitope coverage across the PTPRC extracellular and intracellular domains, which can be advantageous when probing for isoform variants such as CD45RA, CD45RO, and CD45RB generated by alternative splicing of exons 4, 5, and 6.

With confirmed reactivity against human and mouse targets, the antibody enables comparative studies across species, including xenograft and syngeneic tumor models where CD45 staining is routinely used to gate out host immune cells from tumor cell populations. Researchers working on hematological malignancies, autoimmune disease models, and transplant rejection can apply this reagent to characterise leukocyte composition, activation states, and developmental stages in bone marrow, thymus, spleen, lymph node, and peripheral blood preparations.