Anti-PTPN6 Antibody

From the Antibodies.com catalogue, this Rabbit polyclonal reagent is raised against PTPN6 (also known as SHP-1, HCP, SH-PTP1, PTP-1C, PTP1C, and Tyrosine-protein phosphatase non-receptor type 6), a cytoplasmic tyrosine phosphatase implicated in hematopoietic cell signalling. The antibody is generated by immunising rabbits with a recombinant human PTPN6 protein and is subsequently affinity-purified on the immunogen, yielding a highly specific polyclonal preparation that recognises the endogenous total PTPN6 protein in native samples. Supplied at 1.0 mg/mL in an unconjugated format, the product is presented in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. The 50 µL pack size is shipped at 4 °C; upon arrival the vial should be aliquoted and stored at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. The reagent has been validated for Western blotting and immunocytochemistry/immunofluorescence (ICC/IF), and published data on the Antibodies.com product page further illustrate positive signals across human, mouse and rat samples, including immunohistochemistry of paraffin-embedded oesophageal, thyroid and rat brain tissues. Recommended working dilutions are application-dependent, with a typical 1:200 dilution shown for IHC imaging and matching fluorescence staining on U2OS cells counter-stained with DAPI. Intended for research use only, this Antibodies.com polyclonal antibody is a versatile tool for laboratories investigating PTPN6 function in haematopoietic and immune signalling pathways.