Anti-PTPN6 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets PTPN6 (also known as SHP-1), a cytoplasmic Src-homology-2 domain-containing protein tyrosine phosphatase that serves as a critical negative regulator of hematopoietic cell signaling. Raised in rabbit and supplied as a 100 µl volume of polyclonal antiserum, the reagent recognizes an epitope conserved across human, mouse, and rat PTPN6, supporting cross-species experimental work in immunology and cancer research.

The antibody has been validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications, enabling detection of endogenous SHP-1 expression in cell lysates and tissue sections. PTPN6 migrates at approximately 68 kDa on SDS-PAGE gels, allowing straightforward identification by western blot analysis. As an unconjugated primary reagent, it can be paired with HRP-, fluorophore-, or biotin-labeled secondary antibodies, supporting flexible assay design for signaling studies, immune cell phenotyping, and tumor suppressor investigations in lymphoma and leukemia models where loss of SHP-1 function has been documented.

For storage, refrigerate the vial upon arrival and aliquot for freezing at -20°C if long-term storage is needed; avoid repeated freeze-thaw cycles to preserve antibody activity.