Anti-PTPN11 (phospho Y580) Antibody

PTPN11 encodes the SHP-2 non-receptor protein tyrosine phosphatase, an enzyme that modulates RAS/MAPK and PI3K/AKT signaling downstream of receptor tyrosine kinases. Phosphorylation at tyrosine 580 is recognized as an activating regulatory event that relieves the autoinhibited conformation of the catalytic domain. This unconjugated, rabbit polyclonal IgG reagent from Antibodies.com targets that specific phospho-epitope, allowing researchers to selectively visualize the activated phosphatase pool rather than total SHP-2 levels in fixed tissue sections.

Validated for immunohistochemistry on human, mouse, and rat samples, the antibody is supplied as 200 µl of ready-to-use or dilutable reagent depending on the chosen protocol. Common applications include mapping receptor tyrosine kinase signaling activity in developmental biology, oncology, and studies of RASopathies such as Noonan and LEOPARD syndrome, where germline PTPN11 mutations drive aberrant phosphatase function. It is also used to investigate cytokine receptor, growth factor, and integrin-linked signaling events in paraffin-embedded or frozen sections, and to assess whether phosphatase engagement in tumor microenvironments correlates with downstream effector activation.

The reagent is provided as a polyclonal antiserum fraction from a rabbit host, unconjugated for downstream flexibility with enzyme- or fluorophore-based detection chemistries that the laboratory selects for each IHC workflow. Storage at -20 °C in single-use aliquots preserves phospho-specific binding activity and minimizes freeze-thaw damage to the immunoglobulin. For research use only.