Anti-PTK2 (phospho Y925) Antibody

Phosphorylation of focal adhesion kinase (PTK2/FAK) at the Y925 residue sits at a regulatory node where integrin signaling meets growth-factor-driven mitogenic pathways. This rabbit polyclonal IgG preparation from Antibodies.com specifically detects the phosphorylated Y925 epitope rather than the unmodified protein, allowing researchers to track activation state rather than mere expression level. Western blot on human-derived lysates is the validated application, with the unconjugated format leaving the antibody compatible with HRP, fluorescent, or biotin secondary detection chemistries of the user's choosing.

The 200 µl liquid aliquot is built around the polyclonal response, which spreads epitope recognition across multiple determinants on the phosphorylated C-terminal region surrounding Y925. That breadth helps when the target band shows reduced signal because of nearby post-translational modification or differences in sample preparation between cell lines.

Because reactivity has been characterized against human PTK2, the reagent is appropriate for signaling studies in adherent human cell models, tumor lysate surveys of focal-adhesion components, and any workflow where the phospho-Y925 site is the readout of choice. Researchers assessing SRC-family kinase coupling, integrin engagement kinetics, or anti-invasive compound screening will find the phospho-specific readout more informative than total FAK levels.