Anti-PTK2 (phospho Y576 + Y577) Antibody

This rabbit polyclonal antibody from Antibodies.com selectively detects PTK2 (also known as Focal Adhesion Kinase, FAK) only when phosphorylated at both tyrosine residues Y576 and Y577. These two sites sit within the activation loop of the FAK kinase domain, and their dual phosphorylation, mediated largely by Src-family kinases, is required for maximal catalytic activity. The reagent is supplied as a 50 µL vial of unconjugated IgG and is validated for Western blot detection of endogenous human PTK2, making it a useful tool for monitoring activation-state changes downstream of integrin clustering, growth factor stimulation, or oncogenic Src signaling in cell-based and biochemical studies. Researchers commonly deploy this antibody when investigating focal adhesion dynamics, cell migration, mechanotransduction, and tumor invasion pathways where FAK activity is a critical readout, since the Y576/Y577 phospho-state distinguishes catalytically engaged FAK from the inactive pool. As an unconjugated reagent, it can be paired with a wide selection of secondary antibodies for chemiluminescent Western blot workflows and is reactive against human PTK2.