Anti-PTK2 Antibody

This rabbit polyclonal antibody targets PTK2 (Protein Tyrosine Kinase 2), commonly known as Focal Adhesion Kinase (FAK), a non-receptor cytoplasmic tyrosine kinase that localizes to focal adhesions and is activated downstream of integrin engagement. FAK plays central roles in cell adhesion, motility, proliferation, and survival signaling, and its overexpression and constitutive activation are frequently observed in metastatic cancers and in tumors with invasive phenotypes.

Provided by Antibodies.com as a 200 µl unconjugated IgG preparation raised in rabbit, this reagent is validated for Western blot (WB) and immunohistochemistry (IHC) applications. With reactivity confirmed against Human and rat PTK2, the antibody enables detection of endogenous levels of the ~125 kDa FAK protein in cell lysates and tissue sections. The polyclonal format offers broader epitope coverage, increasing the likelihood of recognizing the target even when post-translational modifications or partial degradation are present.

Typical uses include probing FAK phosphorylation dynamics in response to integrin or growth factor stimulation, mapping focal adhesion assembly in cultured cells, and characterizing FAK expression patterns in tumor biopsies and normal tissue archives. The unconjugated format allows researchers to pair the antibody with custom reporter conjugates or signal-amplification systems of their choice.

Storage recommendations for this polyclonal primary antibody generally involve aliquoting to avoid repeated freeze-thaw cycles and storing at -20 °C in a suitable buffer to preserve activity for long-term use. For research use only.