Anti-PTH Antibody

This rabbit polyclonal primary antibody from Antibodies.com targets parathyroid hormone (PTH), the 84-amino-acid peptide regulator of calcium and phosphate homeostasis secreted by chief cells of the parathyroid gland. Investigators working on bone metabolism, chronic kidney disease–mineral bone disorder, primary hyperparathyroidism, and parathyroid gland histology use this reagent to localize and detect PTH expression in human tissue sections and cell preparations.

Validated for Western blot, immunohistochemistry, and immunofluorescence on human samples, the unconjugated IgG is supplied as 100 µL at 1 mg/mL in PBS containing 0.1% sodium azide and 50% glycerol. The glycerol cryoprotectant formulation supports storage at -20 °C with minimal freeze-thaw damage, and the azide preservative keeps the reagent stable for routine bench use during extended staining runs. Because it is unconjugated, the antibody pairs cleanly with HRP-, biotin-, or fluorophore-conjugated secondary reagents chosen by the researcher.

Typical applications include Western blot detection of intact PTH at roughly 9.4 kDa and of pro-PTH fragments in cell lysates; immunohistochemistry on formalin-fixed paraffin-embedded parathyroid adenoma, hyperplasia, and normal gland sections; and immunofluorescence on cultured parathyroid or transfected cells expressing preproPTH. Researchers studying secondary hyperparathyroidism in renal failure or exploring ectopic PTH production by tumors benefit from the polyclonal format, which recognizes multiple epitopes along the hormone, including mid-molecule and C-terminal regions conserved between circulating fragments.