Anti-PTEN Antibody

PTEN (Phosphatase and tensin homolog deleted on chromosome 10) is a lipid phosphatase that serves as a critical negative regulator of the PI3K/AKT/mTOR signaling cascade. By dephosphorylating PIP3 to PIP2, PTEN controls cell cycle progression, survival, and proliferation, and its loss-of-function mutations are widely studied in oncology, including prostate, endometrial, breast, and glioblastoma research, as well as in Cowden syndrome and PTEN hamartoma tumor syndromes.

This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the PTEN tumor suppressor and validated for western blot and immunohistochemistry workflows. The antibody cross-reacts with human, mouse, and rat samples, enabling translational studies across species. Each vial contains 100 µl of affinity-purified polyclonal serum that supports downstream detection with HRP-, fluorophore-, or biotin-conjugated secondary reagents.

Typical applications include profiling PTEN expression in tumor tissue microarrays, validating CRISPR knockouts or knockdowns, assessing downstream AKT phosphorylation status, and monitoring pathway reactivation in preclinical drug studies. The polyclonal format also offers broader epitope coverage, which can be useful when probing for truncated or mutant PTEN variants that monoclonal reagents may miss.