Anti-PTEN (Ab-370) Antibody

Raised in rabbit and affinity-purified against a synthetic peptide spanning residues368–372 (D-V-S-D-N) of human PTEN, this polyclonal reagent from Antibodies.com is supplied as a 50 µl aliquot at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, with no fluorophore or enzyme attached (unconjugated). It is validated for Western blotting and immunohistochemistry on human, mouse, and rat samples and binds the endogenous phosphatase/tensin homolog encoded by PTEN (also known as MMAC1/TEP1), enabling detection of the full-length protein at its predicted molecular weight of approximately 54 kDa. Suggested working ranges on the supplier datasheet are 1:500–1:1000 for Western blot and 1:50–1:100 for IHC, supporting clean band or section staining when the antibody is handled correctly. The product is shipped at 4 °C and should be aliquoted and stored long-term at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity; under these conditions the reagent remains stable for routine research use. Intended strictly for research applications, not for diagnostic or therapeutic work, this Antibodies.com reagent offers a reliable tool for laboratories studying PTEN signalling, tumour-suppressor biology, and downstream pathways such as PI3K/AKT regulation.