Anti-PSMD5 Antibody

A 26S proteasome research tool, this polyclonal IgG supports western blot and immunohistochemistry workflows investigating ubiquitin-dependent protein degradation. Produced in rabbit and antigen affinity purified, the unconjugated reagent detects endogenous levels of total PSMD5 protein, also known as S5b or KIAA0072, a 56 kD non-ATPase subunit of the 19S regulatory lid that gates substrate entry into the proteasome's proteolytic core.

From Antibodies.com, the reagent is supplied as a 100 µl vial in a buffered formulation suitable for immunoblotting of cell and tissue lysates and for staining fixed paraffin-embedded sections. Cross-reactivity has been confirmed with human, mouse, and rat samples, enabling comparative work across species. A predicted band near 56 kDa corresponds to the calculated molecular mass of the 505-amino acid PSMD5 polypeptide, a regulatory subunit that localizes predominantly to the cytosol with additional nucleoplasmic distribution in mammalian cells.

For consistent performance, the vial is shipped at 4 °C; aliquot and store at -20 °C for longer-term use while avoiding repeated freeze-thaw cycles. Each vial of 100 µl supports multiple immunoblot and staining runs, providing a useful reagent for studies of proteasome assembly, ER-associated degradation, and ubiquitin-dependent protein turnover within the regulatory particle.