Anti-PSMC6 Antibody

PSMC6 (Proteasome 26S Subunit, ATPase 6), also known as SUG2 or p42, is an AAA-ATPase subunit of the 26S proteasome that drives ubiquitin-dependent protein degradation in eukaryotic cells. This polyclonal antibody from Antibodies.com is raised in rabbit against the PSMC6 target and has been validated for Western blot and immunocytochemistry applications, with reactivity confirmed in human, mouse, and rat samples.

Supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated antibody is suited for detecting endogenous PSMC6 expression in whole-cell lysates and fixed cultured cells. Within the 26S proteasome holoenzyme, PSMC6 contributes to the ATPase activity that unfolds polyubiquitinated substrates and translocates them into the 20S proteolytic core for cleavage into short peptides.

For Western blot, working concentrations in the 0.5-2 µg/ml range are commonly used, while immunocytochemistry on fixed cells is often performed at 1-5 µg/ml, with titration recommended for each detection system. Store the antibody at -20°C in small aliquots to limit freeze-thaw cycles and protect from light; the 50% glycerol formulation keeps the reagent liquid and stable at this storage temperature. The polyclonal format recognizes multiple epitopes on PSMC6, which can improve signal when target abundance is low or when probing across species.

Common research uses include studies of regulated proteolysis, protein quality control, neurodegenerative disease models, and cell cycle progression where 26S activity shifts. Broad species reactivity enables direct comparison of PSMC6 expression in cultured mammalian cells, primary cultures, and tissue-derived samples without switching primaries.