Anti-PSMB5 Antibody

Raised in rabbit as a polyclonal reagent against the proteasome 20S subunit beta-5, this unconjugated primary antibody targets the chymotrypsin-like catalytic subunit of the constitutive 26S proteasome. Supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol for cryoprotection, the 100 µl aliquot is validated for Western blotting, immunohistochemistry, and immunofluorescence on human, mouse, and rat samples. The PSMB5 subunit harbors the catalytically active Thr1 residue responsible for chymotrypsin-like peptidase activity, and point mutations in this region (notably C49S and A50T) have been reported in multiple myeloma cell lines displaying acquired resistance to bortezomib and other proteasome inhibitors. Researchers studying proteasome biology, MHC class I antigen processing, hematological cancer drug resistance, and protein homeostasis rely on robust detection of endogenous PSMB5 levels. From Antibodies.com, this reagent offers a versatile tool for tracking proteasome subunit expression across tissue types and experimental systems.