Anti-PSMB1 Antibody

This Rabbit polyclonal reagent from Antibodies.com targets PSMB1 (proteasome 20S subunit beta 1), a core component of the constitutive proteasome, and is raised against a recombinant human PSMB1 immunogen. The antibody is affinity-purified using the immunogen and supplied at 1.0 mg/mL in phosphate buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. It is unconjugated and of the IgG isotype, and is shipped at 4°C; upon receipt it should be aliquoted and stored at -20°C, with repeated freeze/thaw cycles avoided to preserve activity. Validated applications on the supplier datasheet are Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF), and the reagent has been confirmed to detect endogenous levels of total PSMB1 protein. The 50 µl pack size is verified for use on Human, Mouse, and Rat samples, making it suitable for cross-species studies of proteasomal function in cell lysates and fixed-cell preparations. As a polyclonal, the antibody binds multiple epitopes on PSMB1, which can improve signal robustness in Western blotting under standard reducing conditions. The product is intended for research use only and is not validated for diagnostic applications, so appropriate secondary antibodies and controls should be selected to match the chosen detection method and species.