Anti-PSMA1 Antibody

The PSMA1 proteasome subunit forms part of the 20S core particle of the constitutive proteasome, a multi-catalytic complex that degrades ubiquitin-tagged proteins in the cytosol and nucleus. This rabbit polyclonal reagent from Antibodies.com is raised against recombinant human PSMA1 and validated for Western blot and immunohistochemistry across human, mouse, and rat samples, making it a versatile reagent for studies of proteostasis, cell cycle regulation, and apoptosis pathways.

The antibody is supplied as 100 µL of unconjugated IgG at 1 mg/ml in PBS buffer containing 0.1% sodium azide and 50% glycerol, which preserves activity during storage at -20 degrees C and limits freeze-thaw damage. Aliquot upon first use if repeated freeze-thaw cycles are anticipated. The polyclonal format detects multiple epitopes on the ~30 kDa PSMA1 alpha ring subunit, increasing the likelihood of recognising the protein after SDS-PAGE transfer even with limited denaturation.

For Western blot, expected band size sits around 30 kDa. For IHC on formalin-fixed paraffin-embedded sections, antigen retrieval in citrate buffer pH 6.0 is commonly applied. The sodium azide in the formulation is incompatible with some live-cell staining workflows and HRP-conjugated secondary detection unless first removed by dialysis or a spin column.