Anti-PRPF3 Antibody

This polyclonal primary antibody from Antibodies.com targets PRPF3 (Pre-mRNA Processing Factor 3), a 77 kDa spliceosomal protein that assembles into the U4/U6.U5 tri-snRNP and is required for catalytic step two of pre-mRNA splicing. The reagent is raised in rabbit and supplied as 100 µl of unconjugated immunoglobulin at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, intended for Western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples.

PRPF3 concentrates in nuclear speckles and Cajal bodies, and dominant mutations in the gene are a known cause of autosomal retinitis pigmentosa type 18, making retinal tissue a major area of interest alongside transformed cell lines such as HeLa and HEK293. Investigators apply the antibody to study spliceosome assembly, confirm loss-of-function phenotypes, and image splicing-factor redistribution under transcription-blocking or heat-shock conditions. The 50% glycerol formulation permits storage at -20 °C; aliquot on first use and limit freeze-thaw cycles to preserve binding activity.