Anti-PRPF18 Antibody

The PRPF18 pre-mRNA splicing factor is targeted by this rabbit polyclonal reagent, raised against the human antigen and validated for both western blot and immunocytochemistry workflows. Antibodies.com formats the antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, supplied as a 100 µl aliquot suited for repeated short-term use.

Reactivity has been confirmed against human, mouse, and rat samples, making the reagent practical for cross-species splicing-factor studies where comparative immunoblotting or fixed-cell imaging is needed. The unconjugated format allows researchers to couple the antibody to their preferred reporter or bead system, including HRP, biotin, or fluorophore conjugations prepared in-lab.

For western blot, the working concentration is diluted from the 1 mg/ml stock, and detection is expected at the endogenous band corresponding to PRPF18. In immunocytochemistry on cultured mammalian cells, the polyclonal host provides signal amplification across the spliceosomal distribution. Sodium azide in the buffer serves as a preservative; care should be taken if combining with azide-sensitive live-cell workflows or secondary reagents.

Storage recommendation: keep refrigerated in the original vial; for longer-term storage, aliquot and freeze to limit freeze-thaw cycles that can degrade IgG over time.