Anti-PROX1 Antibody

PROX1 (Prospero Homeobox 1) is a homeodomain-containing transcription factor that governs lymphatic endothelial cell identity, hepatocyte differentiation during liver organogenesis, and neuronal subtype specification in the developing brain. This rabbit polyclonal IgG reagent from Antibodies.com is supplied as 100 µl of unconjugated antibody, affinity-purified and validated for Western blot (WB), immunohistochemistry (IHC), and flow cytometry (FC) on human and mouse specimens.

Researchers reach for Anti-PROX1 Antibody when they need to map the lymphatic vasculature in tumor microenvironment studies, trace hepatocyte lineage commitment in regenerative biology, or follow neural progenitor fate in developmental neuroscience. Because PROX1 concentrates in the nucleus, the antibody performs well in immunostaining of formalin-fixed paraffin-embedded sections and in intracellular flow cytometry protocols that fix and permeabilize dissociated cells.

The polyclonal host background provides recognition of multiple epitopes on the PROX1 protein, an advantage when probing conserved regions across species. Each vial is intended for research use only; aliquot the antibody upon arrival, refrigerate for routine use, and freeze for long-term storage to limit loss of titre across repeated freeze-thaw cycles. For Western blot, a band near the predicted molecular weight of human PROX1 should be detectable in lysates from tissues or cell lines known to express the transcription factor, while IHC experiments commonly show nuclear staining in lymphatic endothelium and developing lens tissue.