Anti-Proteasome subunit beta type-4 Antibody

This rabbit polyclonal antibody recognizes the 20S proteasome core subunit PSMB4, an approximately 29 kDa catalytic component of the ubiquitin-dependent protein degradation machinery. The reagent from Antibodies.com is supplied at 100 µg, unconjugated, and validated for Western blot and immunohistochemistry on human samples. Endogenous PSMB4 can be detected in a variety of cultured cell lines and tissue specimens, where it contributes chymotrypsin-like activity to the constitutive proteasome beta ring. Researchers studying proteasome assembly, bortezomib-class inhibitor response in multiple myeloma models, or ubiquitin-proteasome pathway dysregulation in cancer biology use this reagent for denaturing immunoblotting of whole-cell lysates and for antigen retrieval-based IHC on formalin-fixed paraffin-embedded sections. The polyclonal rabbit format permits recognition of multiple epitopes on the beta4 subunit, which can help when probing proteasome holoenzyme assembly states or comparing mature versus immature beta-subunit pools. Aliquot on receipt and store at -20 °C to preserve activity through repeated freeze-thaw cycles; the unconjugated format leaves the antibody open to subsequent HRP, fluorophore, or biotin labeling depending on assay design.