Anti-Prostate Specific Antigen (PSA) Total Antibody

Manufactured under the Antibodies.com primary antibody range, this monoclonal reagent targets Prostate Specific Antigen (PSA) Total, the 33–34 kDa kallikrein-family serine protease (KLK3) that is secreted by prostatic epithelial cells and widely used as a serum and tissue marker of prostatic disease. The clone is produced in mouse and is of the IgG1 isotype, provided as an ion-exchange-purified monoclonal antibody in unconjugated form, supplied at a 1 mg pack size for use in research immunoassays. According to the supplier, the antibody has been validated for application in ELISA and Western blotting (WB), and it reacts with human prostate-specific antigen, recognising normal prostatic epithelium as well as benign prostatic hyperplasia and malignant prostate carcinoma tissue. Because the antibody recognises an epitope expressed on the PSA molecule, it is suitable for total PSA detection strategies that include both free PSA and PSA-ACT (PSA-alpha-1-antichymotrypsin) complex. As an unconjugated reagent it can be paired by the end user with a labelled secondary antibody or with a biotin/streptavidin detection system, or used as a capture or detection antibody in matched-pair ELISA development. For routine handling the purified antibody should be stored at 2–8 °C; for longer-term storage the supplier recommends freezing at -20 °C, with repeated freeze-thaw cycles avoided. The product is classified as a Primary Antibody, is intended for research use only, and is offered through AFMS as the regional distributor for the Antibodies.com catalogue. As supplied by Antibodies.com, each lot is quality-controlled and the documentation accompanying the vial confirms clonality, isotype, host species, conjugate status and the validated applications so that scientists can integrate the reagent directly into existing WB and ELISA protocols for prostate cancer biomarker research.