Anti-Prostate Specific Antigen (PSA) Free Antibody

The free, unbound fraction of prostate specific antigen (PSA) is a clinically informative analyte, because the free-to-total PSA ratio is used to help distinguish prostate cancer from benign prostatic hyperplasia. Antibodies.com offers this mouse monoclonal reagent raised specifically against that free PSA species, giving assay developers a probe selective for uncomplexed PSA rather than the alpha-1-antichymotrypsin-bound form.

Reagent properties:

• Host: mouse; clonality: monoclonal; isotype: IgG1
• Conjugate: unconjugated, supporting flexible pairing with HRP, biotin, or fluorophore-labeled secondary reagents
• Purification: ion exchange chromatography
• Validated uses: ELISA and Western blot
• Pack size: 1 mg supplied in a vial
• Handling: refrigerate on receipt; aliquot to limit freeze-thaw cycles

As an unconjugated IgG1, this clone can be deployed as either the capture or detection antibody in two-site immunoassays, and on Western blot it reveals the mature PSA band near 30-34 kDa in prostate-derived cell lysates and tissue extracts. Combining it with a second anti-PSA monoclonal that recognizes a non-overlapping epitope typically strengthens assay sensitivity, while researchers investigating prostate cancer biomarkers, kallikrein protease biology, or diagnostic assay standardization will find the free-PSA selectivity particularly relevant.