Anti-Prohibitin Antibody

Highly conserved ~32 kDa mitochondrial chaperone Prohibitin (PHB) is the antigen recognized by this unconjugated rabbit polyclonal reagent, supplied at 100 µg per vial for research use only. The antibody is validated for Western blot detection of human Prohibitin in cell lysates and for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, reflecting PHB's well-documented dual localization to the inner mitochondrial membrane and to nuclear or plasma-membrane compartments. Prohibitin contributes to respiratory-chain complex assembly, modulates estrogen-receptor transcriptional activity, gates the G1/S transition of the cell cycle, and influences apoptosis through interactions with signaling partners such as Raf-1; its altered expression is reported across breast, gastric, colorectal, and prostate cancers, which is why it remains a frequent subject of oncology, mitochondrial-biogenesis, and senescence research. The polyclonal format recognizes multiple epitopes on the antigen, supporting detection in denatured lysates as well as fixed sections, and the unconjugated format lets each laboratory conjugate the reagent to its preferred HRP, biotin, or fluorophore reporter. For long-term stability, aliquot the antibody upon arrival and freeze at -20 degrees C in a manual-defrost freezer, avoiding repeated freeze-thaw cycles to preserve binding activity. The reagent is manufactured by Antibodies.com and is intended for laboratory research applications in academic, biotech, and clinical-investigation settings.