Anti-PROC Antibody

Protein C (PROC) is a vitamin K-dependent plasma zymogen that, once activated by the thrombin–thrombomodulin complex on the endothelial surface, functions as a key natural anticoagulant by proteolytically inactivating Factor Va and Factor VIIIa. It is also implicated in cytoprotective, anti-inflammatory, and anti-apoptotic signaling through protease-activated receptor pathways, and PROC deficiency or dysfunction is strongly associated with venous thromboembolism, neonatal purpura fulminans, and warfarin-induced skin necrosis. This rabbit polyclonal IgG reagent from Antibodies.com was raised against human Protein C and recognizes the endogenous protein in human and rat tissue samples. The unconjugated antibody is supplied as 100 µl of liquid stock, suitable for immunoblotting and immunohistochemistry workflows, and is typically stored aliquoted at -20 °C to preserve activity over multiple freeze-thaw cycles. In Western blot, it detects the PROC band in plasma and cell lysates under reducing conditions, while in IHC it produces cytoplasmic staining in hepatocytes and vascular endothelium, where Protein C is synthesized and acts, respectively. The polyclonal format increases the likelihood of robust recognition even if a single linear epitope is masked by formalin fixation, and cross-reactivity with rat tissue extends its utility to preclinical thrombosis and vascular-injury models. Researchers studying hemostasis, sepsis-associated coagulopathy, liver-directed protein expression, or the interaction between coagulation and innate immunity can use this reagent to map Protein C distribution and to monitor expression changes in disease models and gene-expression studies.