Anti-PRMT5 Antibody

The Anti-PRMT5 Antibody from Antibodies.com is a rabbit polyclonal IgG reagent targeting protein arginine methyltransferase 5 (PRMT5), a ~72 kDa type II enzyme that catalyzes symmetric dimethylation of arginine residues on histone and non-histone substrates. In western blot (WB) and immunocytochemistry (ICC) workflows, the antibody detects endogenous PRMT5 in human and mouse samples, supporting studies of spliceosome assembly via Sm protein methylation, transcriptional regulation through H3R2me2s and H4R3me2s chromatin marks, and the Golgi-associated cytoplasmic pool of the enzyme.

The unconjugated reagent is supplied as a 100 ug liquid aliquot in a single-use vial and is used in research on cancer cell signaling, hematopoiesis, and stem cell biology, where PRMT5 is frequently upregulated. For best stability, store at -20 degrees C in working aliquots to limit freeze-thaw cycles, and pair with a housekeeping marker such as beta-actin or GAPDH when probing whole-cell lysates by WB.