Anti-PRMT5 Antibody

PRMT5 (Protein Arginine Methyltransferase 5) is a type II protein arginine methyltransferase that catalyzes symmetric dimethylation of arginine residues on histone and non-histone substrates. The Anti-PRMT5 Antibody from Antibodies.com is a recombinant rabbit monoclonal reagent that specifically recognizes this target and is validated across mammalian sample types.

Supplied as 100 µl of unconjugated antibody in a stabilizing buffer of 1×TBS (pH 7.4), 1% BSA, and 40% glycerol with 0.05% sodium azide as preservative, this antibody is validated for Western blot, immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry applications. It cross-reacts with Human, Mouse, and Rat samples, supporting translational and comparative studies.

PRMT5 functions within the methylosome complex alongside MEP50/WDR77 and regulates snRNP biogenesis, pre-mRNA splicing, and epigenetic gene regulation through modification of histone H4R3 and H3R8. Researchers use this reagent to investigate cancer biology, where PRMT5 overexpression is documented in mantle cell lymphoma, glioblastoma, and melanoma, as well as stem cell pluripotency maintenance and splicing factor regulation. Store refrigerated and protect from light; aliquot working stocks to limit freeze-thaw cycles.