Anti-PRMT5 Antibody

This unconjugated polyclonal reagent from Antibodies.com targets PRMT5 (Protein Arginine Methyltransferase 5), a type II arginine methyltransferase that catalyzes symmetric dimethylation of arginine residues on histone and non-histone substrates. The antibody is raised in rabbit and supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2. Validated for western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation workflows, it shows reactivity with both human and mouse samples, supporting studies of transcriptional repression, spliceosomal Sm protein modification, and p53 pathway regulation. Researchers investigating hematological malignancies, lung cancer biology, and germ cell development will find this reagent useful for probing symmetrically dimethylated arginine marks such as H3R2me2s and H4R3me2s, as well as non-histone targets within the broader methylosome complex. The 100 µl volume supports multiple replicate blots or staining runs, and aliquoting upon first use helps preserve the stock against repeated freeze-thaw cycles. Storage at 4°C short term or -20°C long term is recommended, with protection from light to maintain reagent integrity for downstream assay and detection applications.