Anti-PRL Antibody

Produced by Antibodies.com, this rabbit polyclonal reagent recognizes human Prolactin (PRL), the polypeptide hormone secreted by anterior pituitary lactotrophs that regulates lactation and participates in many additional signaling roles throughout the body. The antibody is supplied unconjugated in a 50 µl vial, raised against prolactin in rabbit hosts, and is recommended for Western blot and immunohistochemistry workflows on human samples.

Typical applications include detecting prolactin bands in Western blots of cell lysate or tissue homogenate, where the primary antibody is paired with an HRP-conjugated secondary reagent and developed via chemiluminescent substrate. For immunohistochemistry, paraffin-embedded sections are commonly deparaffinized, rehydrated, and subjected to heat-induced antigen retrieval in citrate buffer (pH 6) before overnight incubation with the primary reagent. In both workflows, chromogenic or fluorescence-based detection can be implemented depending on the secondary reagent chosen. Signal is most reliable in tissues known to express prolactin, including anterior pituitary, decidua, and certain breast and prostate specimens.

The reagent is for research use only and is not approved for diagnostic procedures. To preserve activity, the contents should be aliquoted upon first thaw and stored frozen at -20°C for routine use, or at -80°C for longer-term storage; repeated freeze-thaw cycles should be avoided by preparing working stocks in single-use volumes. Investigators studying pituitary adenomas, reproductive endocrinology, lactation biology, or the extrapituitary roles of prolactin will find this polyclonal reagent a flexible tool for both protein-level detection and tissue-localization studies.