Anti-PRKCZ (phospho T410) Antibody - Identical to Abcam (ab194922)

This rabbit polyclonal IgG antibody specifically recognizes Protein Kinase C Zeta (PRKCZ/PKCζ) when phosphorylated at threonine 410, a residue within the kinase activation loop whose phosphorylation is required for full catalytic activity downstream of PI3K and Par-3/Par-6 polarity signaling. Supplied by Antibodies.com as a 200 µl unconjugated vial, the reagent is validated for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections and shows reactivity across Human, Mouse, and Rat samples, enabling comparative studies of atypical PKC pathway activation in normal and diseased tissue.

The unconjugated format allows pairing with HRP- or fluorophore-conjugated anti-rabbit secondary reagents, supporting chromogenic detection, immunofluorescence, and tyramide signal amplification workflows. The polyclonal nature of the reagent means recognition of multiple epitopes on phospho-PRKCZ, which can improve signal in IHC when antigen is limited. For storage, keep the vial at -20 °C, avoid repeated freeze-thaw cycles by aliquoting on first use, and protect from light. Researchers investigating NF-κB activation, insulin signaling, cell polarity, or tumor pathway dysregulation will find this phospho-specific reagent useful for mapping T410 phosphorylation status in situ at single-cell resolution.