Anti-PRKCD (phospho S645) Antibody

Phospho-specific detection of Protein Kinase C delta (PKCδ) at the activating serine 645 autophosphorylation site is enabled by this affinity-purified rabbit polyclonal reagent from Antibodies.com. Raised against a synthetic phosphopeptide corresponding to residues flanking S645 of human PRKCD, the unconjugated IgG selectively recognizes the phosphorylated kinase while showing minimal cross-reactivity with the unphosphorylated form.

Supplied as a 200 µl vial, the antibody is validated for western blot detection of the ~78 kDa PKCδ band in lysates from human, mouse, and rat origin, and for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. Investigators studying apoptosis, oxidative stress responses, or receptor-driven signaling cascades apply the reagent to monitor PKCδ activation status across cell culture and primary tissue models.

For storage, hold the antibody at -20 °C in small working aliquots and limit freeze-thaw cycles to preserve phospho-selective binding. Each lot is quality-controlled by western blot against stimulated versus untreated controls.