Anti-PRKAA1 Antibody

PRKAA1 encodes the catalytic alpha-1 subunit of AMP-activated protein kinase (AMPK), a heterotrimeric serine/threonine kinase that acts as a central cellular energy sensor. When cellular AMP rises and ATP falls, AMPK restores energetic homeostasis through catabolic pathways such as glucose uptake and fatty acid oxidation while repressing anabolic lipid and protein synthesis. This rabbit polyclonal reagent from Antibodies.com is raised against PRKAA1 and is supplied as 100 µl of unconjugated antisera, formatted in a vial ready for direct dilution into standard immunodetection workflows.

The antibody has been validated for western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation in human, mouse, and rat samples, providing a flexible tool for studies of metabolism, autophagy, mitochondrial biogenesis, and insulin signaling. The polyclonal format recognizes multiple epitopes on PRKAA1, which can be advantageous when probing post-translational phosphorylation events or conformation-dependent variants that a single monoclonal clone may miss. Endogenous expression profiling in primary cells, tissue sections, and lysates is supported by the unconjugated presentation, allowing choice of HRP, biotin, or fluorophore secondary reporters tailored to each application. For optimal storage, keep the vial at -20°C in small working aliquots to limit freeze-thaw cycles and protect from light; avoid sodium azide when downstream immunofluorescence work demands live-cell compatible preparations.