Anti-Prion protein PrP Antibody [2C5-5]

The 2C5-5 monoclonal antibody targets a conformational epitope on the human prion protein (PrP), binding within the globular domain of the cellular isoform PrPC. This reagent from Antibodies.com is supplied as a 50 µg liquid preparation in an IgG1 format, presented in a sealed vial and validated for Western blot and ELISA workflows investigating transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease and variant CJD, as well as bovine spongiform encephalopathy and scrapie models.

Researchers commonly use 2C5-5 to probe tissue homogenates, transfected cell lysates, and recombinant PrP preparations, supporting studies on protein misfolding, aggregation kinetics, and the cellular trafficking of normal cellular prion protein. The unconjugated mouse IgG1 format allows flexible pairing with secondary reagents and custom labelling strategies, including chromogenic, fluorescent, and HRP-conjugated reporters.

For best results, store the antibody frozen at -20 °C in working aliquots, avoiding repeated freeze-thaw cycles to preserve binding activity. Each vial is supplied at approximately 1 mg/mL concentration suitable for direct dilution into standard immunoblotting and plate-coating buffers. The reagent shows reactivity specifically with human PrP, enabling selective detection in patient-derived samples and engineered cell line models expressing wild-type or mutant prion protein.