Anti-PRDX5 Antibody

This Rabbit polyclonal reagent from Antibodies.com targets PRDX5 (Peroxiredoxin 5), a mitochondrial and peroxisomal antioxidant enzyme, and is supplied as a 50 µl aliquot at a concentration of 1.0 mg/mL. The antibody is unconjugated and was raised against a recombinant human PRDX5 immunogen, then purified by affinity chromatography using that same immunogen, yielding an IgG polyclonal preparation in a PBS-based buffer (pH 7.4, 150 mM NaCl) containing 0.02% sodium azide and 50% glycerol as a cryoprotectant. The reagent is validated for Western blotting (recommended dilution 1:500–1:2000) and for immunofluorescence on cultured cells (ICC/IF, 1:50–1:200), and it shows cross-reactivity with Human, Mouse, and Rat samples, detecting the endogenous total PRDX5 protein in each of these species. For handling, the antibody is shipped at 4 °C and should be aliquoted upon arrival and stored long-term at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity, while continuous-use aliquots can likewise be kept at –20 °C. The combination of affinity-purified polyclonal specificity, broad mammalian reactivity, and a flexible buffer compatible with both membrane-based and imaging workflows makes this Antibodies.com product suitable for routine detection of PRDX5 in expression, localization, and oxidative-stress-related studies.